About Us  |  feedback  |  Contact Us   
Applications Notes                                                                  
Improved sequence coverage and confident protein identification using a two-step digestion protocol combined with acid-labile detergent


This application note clearly demonstrates the benefits of using the LPI™ FlowCell in a two-step digestion protocol using a shave-and-conquer approach. New peptides are identified in the second digestion step leading to improved sequence coverage, an increased number of confident protein identifications and a completely new set of proteins not detected in the first digestion step. Membrane protein vesicles are immobilized on the surfaces of LPI™ Maxi FlowCell, where the flow cell design allows for easy exchange of solutions containing e.g. proteases. This feature is highly suitable for obtaining multiple complementary peptide fractions from a single sample, greatly enhancing the protein sequence information obtainable from a membrane preparation analysis.

Download summarized Application Note
Download detailed Application Note

Arabidopsis thaliana: Plesma membrance Protein analsis
Using LPI FlowCell


This study demonstrates the use of Nanoxis LPI™ FlowCell in conjunction with LC-MS/MS to profile the protein content of a plasma membrane preparation derived from Arabidopsis thaliana, with respect to identity, membrane association types and subcellular locations. A total of 249 proteins were identified, there of 138 proteins (55%) were classed as membrane associated. Subcellular localization analysis of the membrane proteins revealed that the dominant membrane compartment represented was the plasma membrane (88).

Read summarized Application Note
Download detailed Application Note