Abstract

This application note clearly demonstrates the benefits of using the LPI™ FlowCell in a two-step digestion protocol using a shave-and-conquer approach. New peptides are identified in the second digestion step leading to improved sequence coverage, an increased number of confident protein identifications and a completely new set of proteins not detected in the first digestion step. Membrane protein vesicles are immobilized on the surfaces of LPI™ Maxi FlowCell, where the flow cell design allows for easy exchange of solutions containing e.g. proteases. This feature is highly suitable for obtaining multiple complementary peptide fractions from a single sample, greatly enhancing the protein sequence information obtainable from a membrane preparation analysis.

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