Normal Human Primary Cell Lysate

In the past few years, micro RNA with 15 - 30 nucleotides in length have been shown biologically significant as ubiquitous, versatile repressors of gene expression. ScienCell Research Laboratories' micro RNA is isolated from a wide variety of normal human cell types by commercial available isolation kit, which employs an organic extraction followed by immobilization of RNA on glass-fiber filters to enrich micro RNA. The micro RNA products are delivered in DEPC treated water.

Quality Control

The A260/280 ratio of the micro RNA is measured to be ~2.0 using Beckman Coulter DU 800 spectrophotometer.
The integrity of micro RNA is checked by electrophoresis on 15% denaturing polyacrylamide gel. The 5.8S rRNA, 5S rRNA and tRNA species are efficiently recovered.

Applications

RT-PCR
Blot hybridization .
Solution hybridization assays .
RNA amplification and microarray analysis .

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